TMPC Publications

Aceti DJ, Bingman CA, Wrobel RL, Frederick RO, Makino SI, Nichols KW, Sahu SC, Bergeman LF, Blommel PG, Cornilescu CC, Gromek KA, Seder KD, Hwang S, Primm JG, Sabat G, Vojtik FC, Volkman BF, Zolnai Z, Phillips GN Jr, Markley JL, Fox BG. (2014) Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies.. J Struct Funct Genomics, in press. PMID:25854603 / PMCID journal - in process

Aly KA, Beebe ET, Chan CH, Goren MA, Sepúlveda C, Makino SI, Fox BG, Forest KT (2012) Cell-free production of integral membrane aspartic acid proteases reveals zinc-dependent methyltransferase activity of the Pseudomonas aeruginosa prepilin peptidase PilD. MicrobiologyOpen 2(1):94-104. PMID:23255525 / PMCID:PMC3584216

Beebe ET, Makino SI, Markley JL, Fox BG (2014) Automated cell-free protein production methods for structural studies. Methods Mol Biol 1140:117-35. PMID: 24590713 / PMCID - Policy Exempt

Cao Y, Aceti DJ, Sabat G, Song J, Makino SI, Fox BG, Bent AF (2013) Mutations in FLS2 Ser-938 dissect signaling activation in FLS2-mediated Arabidopsis immunity. PLoS Pathog 9(4): e1003313. PMID:23637603 / PMCID:PMC3630090

Gromek KA, Meddaugh HR, Wrobel RL, Suchy FL, Bingman CA, Primm JG, Fox BG (2013) Improved expression and purification of sigma 1 receptor fused to maltose binding protein by alteration of linker sequence. Prot Expr Purif 89(2):203-9. PMID:23562661 / PMCID:PMC3679933

Gromek KA, Suchy FL, Meddaugh HR, Wrobel RL, LaPoint L, Chu UB, Primm JG, AE Ruoho, Senes A, Fox BG (2014) The oligomeric states of the purified sigma 1 receptor are stabilized by ligands. JBC, 289(29):20333-20344. PMID:24847081 / PMCID:PMC4106346

Jarecki BW, Makino SI, Beebe ET, Fox BG, Chanda B (2013) Function of Shaker potassium channels produced by cell-free translation upon injection into Xenopus oocytes. Scientific Reports 3:1040. PMID:23301161 / PMCID:PMC3539143

Makino SI, Beebe ET, Markley JL, Fox BG (2014) Cell-free protein synthesis for functional and structural studies. Methods Mol Biol 1091:161-78. PMID:24203331 / PMCID - Policy Exempt

Pieper U, Schlessinger A, Kloppmann E, Chang GA, Chou JJ, Dumont ME, Fox BG, Fromme P, Hendrickson WA, Malkowski MG, Rees DC, Stokes DL, Stowell MHB, Wiener MC, Rost B, Stevens RC, Stroud TM, Sali I (2013) Coordinating the impact of structural genomics on the human α-helical transmembrane proteome. Nat Struct Mol Bio 20(2):135-8. PMID:23381628 / PMCID:PMC3645303

Takasuka TE, Walker JA, Bergeman LF, Vander Meulen KA, Makino SI, Elsen NL, Fox BG (2014) Cell-free translation of biofuels enzymes. Methods Mol Biol 1118:71-95. PMID:24395410 / PMCID - Policy Exempt

 

Relevant CESG Publications (from PSI-I and PSI-II)

Beebe ET, Makino SI, Nozawa A, Matsubara Y, Frederick RO, Primm JG, Goren MA, Fox BG (2010) Robotic large-scale application of wheat cell-free translation to structural studies including membrane proteins. N Biotechnol 28(3):239-49. PMID:20637905 / PMCID:PMC3035758

Blommel PG, Fox BG (2007) A combined approach to improving large-scale production of tobacco etch virus protease. Protein Expr Purif 55(1):53-68. PMID:17543538 / PMCID:PMC2047602

Blommel PG, Martin PA, Seder KD, Wrobel RL, Fox BG (2009) Flexivector cloning. Methods Mol Biol 498:55-73. PMID:18988018 / PMCID - Policy Exempt

Chae PS, Rasmussen SG, Rana RR, Gotfryd K, Chandra R, Goren MA, Kruse AC, Nurva S, Loland CJ, Pierre Y, Drew D, Popot JL, Picot D, Fox BG, Guan L, Gether U, Byrne B, Kobilka B, Gellman SH (2010) Maltose-neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins. Nat Methods 7(12):1003-8. PMID:21037590 / PMCID:PMC3063152

Chang Y, Mead D, Dhodda V, Brumm P, Fox BG (2009) One-plasmid tunable co-expression for mycobacterial protein-protein interaction studies. Protein Sci 18(11):2316-25. PMID:19760663 / PMCID:PMC2788286

Chang Y, Wesenberg G, Bingman CA, Fox BG (2008) In vivo inactivation of mycobacterial integral membrane stearoyl-CoA desaturase DesA3 by a C-terminal specific degradation process. J Bacteriol 190(20):6686-96. PMID:18723625 / PMCID:PMC2566213

Goren MA, Fox BG (2008) Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex. Protein Expr Purif 62(2):171-8. PMID:18765284 / PMCID:PMC2586813

Goren MA, Fox BG, Bangs JD (2011) Amino acid determinants of substrate selectivity in the Trypanosoma brucei sphingolipid synthase family. Biochem 50(41):8853-61.PMID:21899277 / PMCID:PMC3212988

Goren MA, Nozawa A, Makino S, Wrobel RL, Fox BG (2009) Cell-free translation of integral membrane proteins into unilamelar liposomes. Methods Enzymol 463:647-73. PMID:19892197 / PMCID - Policy Exempt

Makino S-i, Goren MA, Fox BG, Markley JL (2010) Cell-free protein synthesis technology in NMR high-throughput structure determination. Methods Mol Biol 607:127-47. PMID:20204854 / PMCID - Policy Exempt

Sobrado P, Goren MA, James D, Amundson CK, Fox BG (2008) A Protein Structure Initiative approach to expression, purification, and in situ delivery of human cytochrome b5 to membrane vesicles. Protein Expr Purif 58(2):229-41. PMID:18226920 / PMCID:PMC2277500